![]() chili powder: gives the rim a nice spicy little kick!.salt: I suggest using coarse sea salt here, but any coarse salt will work.ice: gotta have crushed ice – or at least some basic ice cubes – to fill those glasses!.orange liqueur: I love Grand Marnier or cointreau, but triple sec will work if it’s all you have.pineapple juice: if you can find it, I suggest using pure pineapple juice, not from concentrate.tequila: blanco tequila works best! not a tequila fan? rum makes a delicious substitute!.buy a couple of extras if you want some for garnish! ![]() limes: you’ll need plenty of limes for this recipe! you’ll use the skin of the limes in the simple syrup, and you’ll use the juice in the margaritas.do not sub pickled jalapeños! in a pinch, use fresh serrano peppers instead. jalapeño peppers: fresh jalapeños work best.water: don’t reduce the amount or sub another liquid here.granulated sugar: brown sugar can be used instead!. ![]() That way once my guests arrive, all I have to do is fill some glasses with ice and serve! This is one of my favorite cocktail recipes to make a couple of hours before a party. Unlike traditional margaritas that need to be individually shaken, these are whipped up in a pitcher. These made-from-scratch spicy margaritas are super refreshing and easily serve a crowd. Question: What’s spicy and sweet… and the perfect weekend cocktail treat?Īnswer: SPICY PINEAPPLE MARGARITAS! An elevated version of my favorite quick cocktail: tequila and pineapple juice. Garnish with fresh pineapple and lime wedges! Next time you need cocktails for a crowd, skip the cocktail shaker and whip up a batch of these pineapple jalapeño pitcher margaritas instead! The simple syrup is cooked with fresh jalapeño slices, which gives these margs a great kick of heat.
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![]() Sometimes you can just go on a frenzy and attack anything. If you have a broadsword and you’re going against an opponent with a dagger, you may want to re-think your choice. Now bear in mind, this game doesn’t necessarily require skill. You can still run in swinging, it’s actually pretty funny given the new animations and art style.Ī little thinking before you strike though can make a difference. Instead of running in flailing swords, the game wants you to occasionally take your time. Nidhogg 2 sets itself apart from it’s former by being more of a thinking based game. Changing the moves allows for more sword-based combat instead of button mashing. It’s not as easy to spam moves anymore and this is accompanied by the alterations to the move-sets. The developers have solved the spamming problem of most players in the original. The controls are tight and responsive which makes a lot of difference when doing rolls or flips. The game comes with 10 levels and each is unique in it’s own way. Utilizing moves at the right time to kill your opponent is often satisfying as it was in the original. Nidhogg’s swordplay is an all-around great feature. Some fans have been forced to change up their routine for taking down an opponent. ![]() Which is a bit of a concern within the community of Nidhogg 1 fans, as their win strategies have now been altered. The moves of Nidhogg 2 have also undergone a transformation. The new weapons feel right for the game and well thought out. There is a feature to turn off weapons that you don’t want to use as to prevent any unlikable selections. There are also new weapons, some weapons are better than others and some require a bit of skill to truly master. At first I wasn’t a big fan of the style, mainly because I was a fan of the original Nidhogg but after awhile it grew on me. For one, the art style is more detailed and colorful. There’s been a few changes since the original Nidhogg. A hilariously fun game, Nidhogg 2 is only complimented by the art style. ![]() Simple, that’s the core belief of Nidhogg created by developer Messhof. Heck, buy both - the Nidhogg Complete Bundle is discounted too.Editor’s Note – This game was reviewed on the Playstation 4 Pro with a review copy being provided by Gaming Instincts. Its new updates are a little smaller than those granted its sequel, understandably, but I'm still glad to have a new battleground called "Fields", a new "Permanent Blood" mode, and those cats.īoth games are also currently 60% off, meaning Nidhogg is £2.79/€4 and Nidhogg 2 is £4.39/€6. I've played it for hours and hours and hours with friends and I never grew tired of it. It's a sword-duelling game that produces exceptionally tense standoffs and intense rivalries, with only a handful of moves or systems to get between you and raw competition. I'm also inclined to agree with Adam in his Nidhogg 2 review, who felt that the sequel's new weapons and larger scope only distracted "from the perfection of the central fencing mechanic."Īnd Nidhogg 1 is perfection. Because it had one minigame that took place on the roof of a train, see. I'm old enough to find the train level reminiscent of Cowboyana, Messhof's old, free western. The former is a "demonic meat locomotive," while the latter is supposedly based on "the real Messhof office." I question this. The two new levels for Nidhogg 2, as explained in a brief Steam post, are "Train" and "Office". Even better news: the original Nidhogg also received a free update, adding a new battleground and some new animated cats. Good news! Chaotic multiplayer brawler Nidhogg 2 just received a free update adding new costumes and two new levels, including one set upon a train. She mentions that she might be able to give you a nice reward if you're able to make the football team. You tell her that you'll do your best and thank her for training you. She says that the next step is for you to apply for the football team. She remarks that she's impressed with your progress, and there's not much more she can teach you. Meet Erika at the pool (afternoon) įind Erika at the school swimming pool again in the afternoon and attend swimming practice. This is the most difficult challenge and the vanilla time limit leaves almost no room for error.ĩ. Talk about motivation!įor this running challenge, there are hurdle obstacles throughout, and the stars are scattered left and right of the center line in addition to being in the center as well. As a reward, Erika promises to flash you by taking off her panties. Meet Erika at running tracks in the morning įind Erika in the same spot at the running tracks and start the next running challenge. She notices it, gets upset, and you hurry off to change.ħ. She'll tackle you to try and put you in your place, and unwittingly gives you an erection. ![]() You'll do better this time, and Erika will be confused as to how you were able to catch up to her. Go to the Swimming Pool again in the afternoon and attend swimming practice. She is flustered but agrees to flash show them to you if you can beat the challenge.Ĭomplete the challenge and Erika will lead you behind some bushes and flash her panties.Ħ. You mention how her coaching style is not very motivating, and rather than just training for training's sake, you'd be motivated if you could see her panties. Go to the Running Tracks again and Erika will mention that the first challenge was only a warm up. Meet Erika at running tracks in the morning After cleaning off and getting dressed, she tells you to come to the Running Tracks again in the morning.Ĥ. You shower at the same time because the concept of taking turns in a community shower wasn't invented until the early 90s, and you sneak a peak at Erika during the shower. Talking with them will reveal that the girls locker room is closed, and that all students will have to share the boys locker room.Īfter some complaining, Erika reluctantly goes into the boys locker room to shower and get dressed, with you close behind. ![]() Walk outside the pool area and you'll see Erika talking with a construction worker outside the girls locker room. After practice, Erika will leave, saying that she's going to hit the showers. Go to the Beagleton Academy Swimming Pool in the afternoon and attend swimming practice with Erika. Reminder: a dose of Vitamin XXX can only be used once, so buy multiple doses or save / reload before attempting the challenge.ģ. There are no obstacles, and all the stars are on the center line. The first running challenge requires you to run around the track once, gathering all the stars on the ground. ![]() Talk to her and if you say that you're ready she'll get the first running challenge set up. Go to the Running Tracks in the morning and you'll find Erika by the 50 yard line on the south side of the field. It can only be used once, so if you only have one dose, you can only use it for one attempt. ![]() This handy 'vitamin' will give you extra time. If you have Vitamin XXX in your inventory, you'll be prompted to use it before beginning the challenge. You promised to train with Erika to be the new wide receiver! In fact, in our playthrough, we won every hole until the seventh. Thankfully, Luigi and DK aren’t the best putters, so even if you aren’t sinking birdies left and right, you should be able to best their time. It’s a par three without any weather, so you should be able to get a quick birdie if you play it safe. The first few holes are your best bets for building up a lead, especially the second hole. That means shooting quickly and accurately is going to be of utmost importance. This round is points-based, so you’ll only score top points if you place first on the hole. You can’t actually go to Mount Snow just yet, so make your way back to the clubhouse and take on the special match to earn your Gold Badge.įor this speed golf match, you’ll be facing off against Luigi and Donkey Kong. Follow your marker through the Wildweather Woods’ clubhouse until the game stops you from progressing. Next, you’ll be sent back to the Balmy Dunes to search for the Power of Flame. You’ll also receive a lengthy exposition dump that explains why all the strange weather is happening at the different courses. Do this three times before the time limit is up and you’ll clear the fight.Īs your reward, you’ll get the Lightning Sword. He’ll toss a lightning ball at you and you need to use duff gauge timing to hit it back toward him. Once this phase is over, go back to one of the mounds his storm cloud kicked up, and press A. He both sends out a storm cloud to light up the ground and shoots beams of lightning at you. When the fight begins, you need to run away from the knight’s lightning attacks. Your final test is a boss fight against the knight statue. ![]() Also, the middle shot is much easier if you use a super lob shot because that shot doesn’t carry as far. The game automatically switches you back to a standard shot, so if you’re not paying attention, it’s easy to fail on accident. It’s important to note that you need to switch to a lob shot after every shot. This test requires you to hit three lob shots in a row over a water wall and into targets. ![]() ![]() Make sure you have the lob wedge equipped or you won’t be able to start this challenge. After you clear the challenge, head forward to your second test. The myapp1 container can then connect to the For example:ĭocker run -name=mysql1 -network= my-custom-net -d /mysql/community-server docker run -name=myapp1 -network= my-custom-net -d myapp Then, when you are creating and starting the server and theĬlient containers, use the -network option to d /mysql/community-server: tag Connect to MySQL from an Application in Another Docker Containerīy setting up a Docker network, you can allow multiple DockerĬontainers to communicate with each other, so that a clientĪpplication in another Docker container can access the MySQL mount type=bind,src= /path-on-host-machine/scripts/,dst=/docker-entrypoint-initdb.d/ \ Into a host directory and then mount the directory at Running Additional Initialization Scriptsĭatabase immediately after it has been created, you can put them Required are mounted when starting the container. It however, you must make sure you start the DockerĬontainer with the same configuration as the server thatĬreated the data, and any host files or directories You can also mount aĭirectory prepopulated with data and start the server with You can also include other server configuration options in Must already exist, and it must contain the specification Server configuration file inside the container), and mount type=bind,src= /path-on-host-machine/datadir,dst=/var/lib/mysql \ mount type=bind,src= /path-on-host-machine/my.cnf,dst=/etc/my.cnf \ ![]() The following commandĬreates a MySQL Server container and bind-mounts both the dataĭirectory and the server configuration file: var/lib/mysql, the server data directoryĪ host directory using the -mount option whenĬreating the container. In which data is persisted on the host, has been mounted at The output shows that the source directory Mount key, whose value provides information Inspect command on the container includes a Server container creates a Docker volume for the server dataĭirectory. Volumes provides a mechanism to persist data created Persisting Data and Configuration Changesĭocker containers are in principle ephemeral, and any data orĬonfiguration are expected to be lost if the container is Persisting Data and Configuration Changes for When you start the MySQL Docker container, you can passĬonfiguration options to the server through the dockerĭocker run -name mysql1 -d /mysql/community-server: tag -character-set-server=utf8mb4 -collation-server=utf8mb4_colĪnother way to configure the MySQL Server is to prepare aĬonfiguration file and mount it at the location of the serverĬonfiguration file inside the container. Products running in such an altered container, or a container Oracle does not provide support for MySQL Any software updates or installations users perform to theĭocker container (including those for MySQL components) mayĬonflict with the optimized MySQL installation created by theĭocker image. ![]() But in applications where you don’t know how much data you might be processing, it’s important that your recursive algorithms are tail-recursive, otherwise you’ll get a nasty StackOverflowError. ![]() I use this simple, basic form of recursion when I know that I’m working with limited data sets. For functions without deep levels of recursion, there’s nothing wrong with the algorithms shown in the previous lessons. If the operators stack is not empty, there may be following possibilites. If the character is an operator and operators stack is empty, push operator into operators stack. If the character is an operand, put it into output stack. Scan input string from left to right character by character. You must see something similar to this: Īnd following is the content inside the example. The following algorithm converts infix to postfix. To make sure the Elasticsearch cluster is up and working fine, open the browser at http : / /localhost : 9200. Next, you can start the Elasticsearch cluster by running bin /elasticsearch on Linux and macOS or bin\elasticsearch. Now that you understand what ELK means, let's learn how to configure all three components to your local development environment: Elasticsearchĭownload the Elasticsearch zip file from the official elastic website and extract the zip file contents. Kibana can be used to search, view, and interpret the data stored in Elasticsearch.
![]() They all went to NC State and have been working to build the local hip hop community. Kooley High is a hip hop collective from Raleigh. Indigo de Souza is an Asheville based garage rock band.ģ:30 p.m. It’s one of 10 artist curated stages at the festival this year. The legendary hip hop is not only reuniting for this year’s festival but they also helped curate the stage on Saturday. – Indigo de Souza open an afternoon that was curated by Durham’s Little Brother. Her chillingly confident song “Wasted Youth” was a big hit on WUNC Music.Ģ:30 p.m. As an adult she’s done music fulltime, first with the indie rock band Rilo Kiley and more recently under her own name. As a kid, she appeared in movies like Troop Beverly Hills. – Jenny Lewis is a child actress turned remarkable songwriter. He was 52 when he died earlier this month.Ĩ:40 p.m. Hopscotch organizers are working on a way to celebrate Berman’s life and work during this slot. Berman’s new band Purple Mountains was scheduled to headline the evening. He’s perhaps best known for his hugely influential work with the group Silver Jews. There’s a huge hole in the Hopscotch schedule on Friday night left by the unexpected death of singer/songwriter David Berman. He writes thoughtful, dense songs that are driven by his intricate guitar work.ħ:00 p.m. ![]() – Tyler Ramsey has been writing songs, making music, and raising his family in Asheville since leaving the group Band of Horses. The band is fronted by singers and guitarists Carrie Brownstein (of Portlandia fame) and Corin Tucker (formerly of Heavens to Betsy) and are still making incredibly vital, relevant and noisy indie rock.ĥ:50 p.m. ![]() – Sleater-Kinney came together towards of the end of the riot grrl movement nearly 25 years ago in the Pacific Northwest. His rich, lo-fi sound will fit perfectly with our late summer humidity.ĩ:45 p.m. – Kurt Vile is a remarkable songwriter who’s been releasing music under his own name since leaving the band The War on Drugs a decade ago. She had the office at NPR Music nodding along to her aggressively sleepy sound.ħ:45 p.m. She was invited to play NPR’s Tiny Desk soon after graduating from high school. Snail Mail is the breakout indie rock project of singer/songwriter Lindsey Jordan. Their rowdy brand of rock and roll will remind you of 70’s era Stones.Ħ:30 p.m. After a couple years of gigging around the area, they piled into a van, hit the road, and haven’t looked back since. The Nude Party all met as students in Boone, North Carolina. Here’s a preview of the broadcast:ĥ:30 p.m. On Saturday, the coverage begins at 2 p.m. WUNC Music is broadcasting live from Hopscotch on Thursday and Friday nights and all day Saturday afternoon at. Credit Bryan Regan Hopscotch Music Festival ![]() In no event may MINOTTI or the managers, administrators, partners and/or employees of MINOTTI be held responsible, in any capacity and for any reason, for the functionality of the Website or for any damage arising from the use of, or failure to use, the Website and contents thereof, or from omissions, inaccuracies or mistakes in the contents of the Website. MINOTTI and the managers, directors, partners and/or employees of MINOTTI do not provide any guarantee as to the functionality of the Website, and the accuracy and completeness of the contents published on the Website in addition, MINOTTI hereby reserves the right to modify and update the said information without notice at any time, at the sole discretion of MINOTTI. 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All the contents of the Website shall be deemed to be protected and safeguarded by current provisions concerning copyright and intellectual and industrial property rights. ![]() ![]() So, whether you're looking for a simple shelf or a complete wardrobe system, you'll find the solution to keep your home organized and clutter-free. At IKEA, we believe that storage furniture should be accessible and affordable for everyone. These can be used to store everything from clothing to toys, and they come in a variety of sizes and styles to suit your needs. In addition to our storage furniture, we also offer a variety of boxes and baskets for small storage. Plus, our storage furniture is designed with personalization in mind, giving you the opportunity to add your personal touch and make it truly yours. You can find pieces that match your taste and home decor with different materials, colours, and finishes. We have options to fit every need and every part of your home, from the living room to the bedroom and beyond. ![]() Our storage furniture helps you make the most of your space, whether in a small apartment or a spacious house. With so many options, you'll surely find the perfect solution to fit your space and style. Bob Schmidt shows you how to take a factory built cabinet and some simple hardware to make a bookcase door.00:03 when you are doing any project you kind of l. That's why we offer a wide range of storage furniture, including wardrobes, bookcases, display cabinets, and shelves. Alternatively why not plan a visit to one of our local showrooms.At IKEA, we understand that having the right storage furniture can make all the difference in keeping your home organized and tidy. Plus, it all comes with a 12-year guarantee.īook a Free Design Visit or download or order a brochure today. Sit back and enjoy your new stylish, organised space. Your order will then be tailor-made from quality, sustainable materials at our state-of-the-art facility in the West Midlands.Īfter delivery, your professional installer will install your dream fitted furniture with a perfect, seamless fit. Once you have placed your order, a technical surveyor will visit your home to double-check every detail. They'll show you samples to inspire you, create a 3D illustration, and provide you with a full quotation. Work with your designer in the comfort of your home, to transform your space and maximise your storage. Read Skai's Privacy Policy.īook a free In-Home Design Visit and receive a no-obligation quote with a range of payment options We use Skai cookies to improve our content and ads, to provide social media features, and to analyse our traffic. This cookie helps us better tailor advertising to people who have already visited our website when they are on Pinterest. 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They do not store directly personal information, but are based on uniquely Used by those companies to build a profile of your interests and show you relevant adverts on These cookies may be set through our site by our advertising partners. ![]() ![]() PMID:2422282ĭescribe the original generation of this antibody, its characterisation and its application to immunodetection of DNA-RNA heteropolymer hybrids.R-loops are non-B-DNA structures consisting of an RNA/DNA hybrid and a displaced single-stranded DNA. ![]() Characterization of monoclonal antibody to DNA.RNA and its application to immunodetection of hybrids. In immunocytochemistry (ICC) and immunoprecipitation (IP) analyses, a representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. In a chromatin Immunoprecipitation-sequencing (ChIP-seq) analysis, a representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast (El Hage et al. In a chromatin immunoprecipitation (ChIP) analysis, a representative lot detected increased DNA-RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia et al. For example, in an affinity binding assay (ABA), this antibody was shown to bind the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding (Boguslawski et al., 1986). Key applications for this antibody include: affinity binding assay (ABA), chromatin immunoprecipitation (ChIP), chromatin immunoprecipitation-sequencing (ChIP-seq), dot blot (DB), fluorescent in situ hybridization (FISH), electrophoretic mobility shift assay (EMSA), surface plasmon resonance (SPR), immunofluorescence (IF), immunocytochemistry (ICC) and immunoprecipitation (IP). In addition, this antibody is effective in recognizing RNA-DNA hybridization for microarray studies. Because RNA-DNA hybrids influence genomic instability, this anti-DNA-RNA hybrid (S9.6) antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.Īpplication Notes: DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. They are shown to be resistant to the activity of DNA methyltransferases. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. Their presence is reported to predispose a locus to chromosomal breakage. They are non-canonical nucleic acid structures with transcriptional regulatory functions. DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. (1986), this antibody was reported to bind the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. In the original publication by Boguslawski et al. It is useful in the detection of R-loops and does not cross-react with single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA. Specificity: This antibody shows high specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids. Immunogen: This antibody was generated in BALB/c mouse against S9.6 ΦX174 bacteriophage-derived synthetic DNA/RNA antigen according to the protocol by Boguslawski et al. FleXpress™ High-Throughput Antibody ProductionĪlternative Name(s) of Target: DNA:RNA Duplex DNA-RNA Duplex DNA/RNA Duplex DNA-RNA Hybrid DNA/RNA Hybrid DNA:RNA Hybrid RNA-DNA Duplex RNA/DNA Duplex RNA:DNA Duplex RNA-DNA Hybrid RNA/DNA Hybrid RNA:DNA Hybrid.Immunoglobulin Fc domains (Ig Fc domains).Anti-Non-Human Primate (NHP) Immunoglobulin Antibodies.VivopureX™ Antibodies for In Vivo Research.Recombinant Antibodies from Human Patients. |
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