![]() ![]() PMID:2422282ĭescribe the original generation of this antibody, its characterisation and its application to immunodetection of DNA-RNA heteropolymer hybrids.R-loops are non-B-DNA structures consisting of an RNA/DNA hybrid and a displaced single-stranded DNA. ![]() Characterization of monoclonal antibody to DNA.RNA and its application to immunodetection of hybrids. In immunocytochemistry (ICC) and immunoprecipitation (IP) analyses, a representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. In a chromatin Immunoprecipitation-sequencing (ChIP-seq) analysis, a representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast (El Hage et al. In a chromatin immunoprecipitation (ChIP) analysis, a representative lot detected increased DNA-RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia et al. For example, in an affinity binding assay (ABA), this antibody was shown to bind the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding (Boguslawski et al., 1986). Key applications for this antibody include: affinity binding assay (ABA), chromatin immunoprecipitation (ChIP), chromatin immunoprecipitation-sequencing (ChIP-seq), dot blot (DB), fluorescent in situ hybridization (FISH), electrophoretic mobility shift assay (EMSA), surface plasmon resonance (SPR), immunofluorescence (IF), immunocytochemistry (ICC) and immunoprecipitation (IP). In addition, this antibody is effective in recognizing RNA-DNA hybridization for microarray studies. Because RNA-DNA hybrids influence genomic instability, this anti-DNA-RNA hybrid (S9.6) antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.Īpplication Notes: DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. They are shown to be resistant to the activity of DNA methyltransferases. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. Their presence is reported to predispose a locus to chromosomal breakage. They are non-canonical nucleic acid structures with transcriptional regulatory functions. DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. (1986), this antibody was reported to bind the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. In the original publication by Boguslawski et al. It is useful in the detection of R-loops and does not cross-react with single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA. Specificity: This antibody shows high specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids. Immunogen: This antibody was generated in BALB/c mouse against S9.6 ΦX174 bacteriophage-derived synthetic DNA/RNA antigen according to the protocol by Boguslawski et al. FleXpress™ High-Throughput Antibody ProductionĪlternative Name(s) of Target: DNA:RNA Duplex DNA-RNA Duplex DNA/RNA Duplex DNA-RNA Hybrid DNA/RNA Hybrid DNA:RNA Hybrid RNA-DNA Duplex RNA/DNA Duplex RNA:DNA Duplex RNA-DNA Hybrid RNA/DNA Hybrid RNA:DNA Hybrid.Immunoglobulin Fc domains (Ig Fc domains).Anti-Non-Human Primate (NHP) Immunoglobulin Antibodies.VivopureX™ Antibodies for In Vivo Research.Recombinant Antibodies from Human Patients.
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